Type : Book Chapter; Journal / Book : Biodiversity in Tropical Ecosystems; Pages : 261-288;
Publisher : Today & Tomorrow’s Printers and Publishers, New Delhi; Place Published : India; Edition : 1;
Date : Jun, 2015 ISBN/ISSN : 9788170195139;

Microbial diversity distribution in the lower belt of eastern Himalaya

  • Joshi SR
  • Bhattacharjee K
  • Banerjee A
  • Bareh DA


North-East India a physiographically categorized zone located along the lower belts of eastern Himalayas falls within the realm of one of the mega-biodiversity hotspots of the world. The region is characterized by the presence of pristine natural ecosystems which are yet to be exposed to anthropogenic disturbances. The pristine niches along with some extreme habitats are bound to provide scope for discovery of novel organisms yet to be known to the scientific community and waiting to be bioprospected for human benefits. The undulating topography with great variation in altitude, soil types, vegetation leads to marked variation in edapho-climatic conditions, the main reason behind the richness in diverse groups of flora and fauna provided by a plethora of habitats harbouring a genetic treasure house of plant, animal and microbial resources. Although there have been substantial amount of scientific literature generated on flora and fauna of the region, the microbial documentation and exploration still is very scarce and calls for a concerted attention of microbiologists. The microbial diversity from the diverse ecological niches of the Indo-Burma biodiversity hotspot holds promise for isolation of biotechnologically significant strains as well as novel species, and provide vistas for studies on the microflora of the biodiversity rich region of the lower belt of eastern Himalayas.

Keywords :
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Type : Short Communication; Journal / Book : Current science; Volume : 108; Issue Number : 09; Pages : 1702-1706;
Publisher : Current science; Place Published : India;
Date : May, 2015

Northeast Microbial Database: A web-based databank of culturable soil microbes from Northeast India

  • Joshi SR
  • Banerjee S
  • Bhattacharjee K
  • Lyngwi NA
  • Koijam K
  • Khaund P
  • Devi LS
  • Nongkhlaw FMW


Northeast Microbial Database (NEMiD) is a first of its kind digital database on microbial diversity from North East (NE) India, which provides information on culturable microbes (bacteria, fungi and actinomycetes) isolated from soils of the region (Arunachal Pradesh, Assam, Manipur, Meghalaya, Mizoram, Nagaland, Tripura, Sikkim and parts of North Bengal) distributed across landscape elements (LSE) spread in various ecosystems. The database is based on the primary data generated through survey, isolation and characterization of the microbes during 2008–2012. Details about on a particular microorganism in the database cover aspects of phylogenetic information on taxonomic outline and classification, morphological, biochemical and molecular characterization, general and cultural characteristics, geographical/location information, history of cultures and preservation details. Each microbe is identified based on cultural, biochemical and molecular characteristics leading to its taxonomic position up to the species level along with information on sampling sites comprising altitude, soil type, latitude, longitude, soil temperature, ambient temperature, humidity and soil pH. The database is available on-line at nemid/.

Keywords : Digital database, landscape elements, natural ecosystems, soil microbes
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Type : Full Length Research Article; Journal / Book : The Journal of Microbiology;
DOI : 10.1007/s12275-014-4113-1; Date : Nov, -0001

Evaluation of the Antimicrobial Potency of Silver Nanoparticles
Biosynthesized by Using an Endophytic Fungus, Cryptosporiopsis
ericae PS4

  • Lamabam Sophiya Devi and Santa Ram Joshi


In the present study, silver nanoparticles (AgNPs) with an average particle size of 5.5 ± 3.1 nm were biosynthesized using an endophytic fungus Cryptosporiopsis ericae PS4 isolated from the ethno-medicinal plant Potentilla fulgens L. The nanoparticles were characterized using UV-visible spec-
trophotometer, transmission electron microscopy (TEM), scanning electron microscopy (SEM), selective area electron diffraction (SAED), and energy dispersive X-ray (EDX) spectroscopy analysis. Antimicrobial efficacy of the AgNPs was analyzed singly and in combination with the antibiotic/anti-
fungal agent chloramphenicol/fluconazole, against five pathogenic microorganisms-Staphylococcus aureus MTCC96, Salmonella enteric MTCC735, Escherichia coli MTCC730, Enterococcus faecalis MTCC2729, and Candida albicans MTCC 183. The activity of AgNPs on the growth and morphology of the microorganisms was studied in solid and liquid growth media employing various susceptibility assays. These studies demonstrated that concentrations of AgNPs alone between 10 and 25 μM reduced the growth rates of the tested bacteria and fungus and revealed bactericidal/fungicidal activity of the AgNPs by delaying the exponential and stationary phases. Examination using SEM showed pits and ruptures in bacterial cells indicating fragmented cell membrane and severe cell damage in those cultures treated with AgNPs. These experimental findings suggest that the biosynthesized AgNPs may
be a potential antimicrobial agent

Keywords : silver nanoparticles, biosynthesis, endophyte, Cry- ptosporiopsis ericae, antimicrobial

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Type : Full Length Research Article; Journal / Book : PLoS ONE; Volume : 9; Issue Number : 4; Pages : e94088;
Publisher :; Place Published : USA;
DOI : 10.1371/journal.pone.0094088; Date : Apr, 2014 ISBN/ISSN : 1932-6203;

NEMiD: A Web-Based Curated Microbial Diversity Database with Geo-Based Plotting

  • Kaushik Bhattacharjee
  • Santa Ram Joshi


The majority of the Earth’s microbes remain unknown, and that their potential utility cannot be exploited until they are
discovered and characterized. They provide wide scope for the development of new strains as well as biotechnological uses.
The documentation and bioprospection of microorganisms carry enormous significance considering their relevance to
human welfare. This calls for an urgent need to develop a database with emphasis on the microbial diversity of the largest
untapped reservoirs in the biosphere. The data annotated in the North-East India Microbial database (NEMiD) were obtained
by the isolation and characterization of microbes from different parts of the Eastern Himalayan region. The database was
constructed as a relational database management system (RDBMS) for data storage in MySQL in the back-end on a Linux
server and implemented in an Apache/PHP environment. This database provides a base for understanding the soil microbial
diversity pattern in this megabiodiversity hotspot and indicates the distribution patterns of various organisms along with
identification. The NEMiD database is freely available at

Keywords : Microbial Diversity, Database
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Type : Full Length Research Article; Journal / Book : Proceedings of the National Academy of Sciences, India Section B: Biological Sciences; Volume : 84; Issue Number : 00; Pages : 000-000;
Publisher : Springer-Verlag; Place Published : USA;
DOI : 10.1007/s40011-013-0223-5; Date : Nov, -0001 ISBN/ISSN : 0369-8211;

A Study on Parameters Optimization for Degradation of Endosulfan by Bacterial Consortia Isolated from Contaminated Soil

  • Kaushik Bhattacharjee
  • Subhro Banerjee
  • Lalsiamthari Bawitlung
  • Dinesh Krishnappa
  • S. R. Joshi


Endosulfan, a non-systemic organochlorine  wide range of crops is of environmental concern because of organisms. The present study was aimed to find out the and/or in consortium and optimization of various growth isolates showing significantly higher degradation potential growth. They were identified as Staphylococcus equorum The effect of various parameters (pH, temperature, inoculum and carbon concentration) were also assessed and optimized Staphylococcus and Bacillus strain showed near disappearance incubation. The maximum degradation percentage of shaking conditions of 150 rpm at 30 C at a concentration used in this study. The isolates identified in this studysites.
Keywords : Endosulfan, Persistent organic pollutant, Bioremediation, Bacterial consortium, Biodegradation

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